• 服務熱線

    15021010459
    產品中心
    當前位置:主頁 > 產品中心 > > 其他生物試劑 >WAK AidSeq™ Primer Sets P201

    WAK AidSeq™ Primer Sets P201

    簡要描述:for sequencing DNA products

    • 產品型號:P201
    • 廠商性質:代理商
    • 更新時間:2025-02-13
    • 訪  問  量:1002
    詳細介紹

    AidSeq™ Primer Sets

    Primer pair for sequencing DNA cloned into vectors such as TA cloning vector (Invitrogen) and pGEM (Promega) that contain SP6 and T7 promoter binding sites.

    重慶市華雅干細胞技術有限公司在倉庫備有現貨,歡迎新老客戶:            

    Genhunter products

       DIFFERENTIAL DISPLAY KITS AND SERVICES

     

     

    1.

     RNAimage® Kits

     

    For mRNA Differential Display with three one-base anchored oligo-dT primers and rationally designed arbitrary 13mers.These kits feature the latest generation of isotopic mRNA Differential Display. Each kit contains all the reagents needed for differential display except RNA samples, Taq DNA polymerase, and  α -[33P] dATP. request info

     

    Cat #

    Quantity

    RNAimage Kit 1
    RNAimage Kit 2
    RNAimage Kit 3
    RNAimage Kit 4
    RNAimage Kit 5
    RNAimage Kit 6
    RNAimage Kit 7
    RNAimage Kit 8
    RNAimage Kit 9
    RNAimage Kit 10

    G501
    G502
    G503
    G504
    G505
    G506
    G507
    G508
    G509
    G510

    Kit
    Kit
    Kit
    Kit
    Kit
    Kit
    Kit
    Kit
    Kit
    Kit

     

     

    2.

     RNAspectra Kits

     

    The RNAspectra Kits feature a new generation of Differential Display technology based on fluorescent detection of PCR products instead of radioactivity. It is the first non-radioactive differential display system with similiar sensitivity to that of 33P isotopic labeling.
    Each kit contains all the reagents needed for fluorescent differential display except RNA samples, Taq DNA polymerase, and a fluorescent scanner. request info

     

    Cat #

    Quantity

    RNAspectra Green Kit 1
    RNAspectra Green Kit 2
    RNAspectra Green Kit 3
    RNAspectra Green Kit 4
    RNAspectra Green Kit 5
    RNAspectra Green Kit 6
    RNAspectra Green Kit 7
    RNAspectra Green Kit 8
    RNAspectra Green Kit 9
    RNAspectra Green Kit 10
    RNAspectra Red Kit 1
    RNAspectra Red Kit 2
    RNAspectra Red Kit 3
    RNAspectra Red Kit 4
    RNAspectra Red Kit 5
    RNAspectra Red Kit 6
    RNAspectra Red Kit 7
    RNAspectra Red Kit 8
    RNAspectra Red Kit 9
    RNAspectra Red Kit 10

    F501
    F502
    F503
    F504
    F505
    F506
    F507
    F508
    F509
    F510
    R501
    R502
    R503
    R504
    R505
    R506
    R507
    R508
    R509
    R510

    Kit
    Kit
    Kit
    Kit
    Kit
    Kit
    Kit
    Kit
    Kit
    Kit
    Kit
    Kit
    Kit
    Kit
    Kit
    Kit
    Kit
    Kit
    Kit
    Kit

    Genhunter products

       DIFFERENTIAL DISPLAY KITS AND SERVICES

     

     

    1.

     RNAimage® Kits

     

    For mRNA Differential Display with three one-base anchored oligo-dT primers and rationally designed arbitrary 13mers.These kits feature the latest generation of isotopic mRNA Differential Display. Each kit contains all the reagents needed for differential display except RNA samples, Taq DNA polymerase, and  α -[33P] dATP. request info

     

    Cat #

    Quantity

    RNAimage Kit 1
    RNAimage Kit 2
    RNAimage Kit 3
    RNAimage Kit 4
    RNAimage Kit 5
    RNAimage Kit 6
    RNAimage Kit 7
    RNAimage Kit 8
    RNAimage Kit 9
    RNAimage Kit 10

    G501
    G502
    G503
    G504
    G505
    G506
    G507
    G508
    G509
    G510

    Kit
    Kit
    Kit
    Kit
    Kit
    Kit
    Kit
    Kit
    Kit
    Kit

     

     

    2.

     RNAspectra Kits

     

    The RNAspectra Kits feature a new generation of Differential Display technology based on fluorescent detection of PCR products instead of radioactivity. It is the first non-radioactive differential display system with similiar sensitivity to that of 33P isotopic labeling.
    Each kit contains all the reagents needed for fluorescent differential display except RNA samples, Taq DNA polymerase, and a fluorescent scanner. request info

     

    Cat #

    Quantity

    RNAspectra Green Kit 1
    RNAspectra Green Kit 2
    RNAspectra Green Kit 3
    RNAspectra Green Kit 4
    RNAspectra Green Kit 5
    RNAspectra Green Kit 6
    RNAspectra Green Kit 7
    RNAspectra Green Kit 8
    RNAspectra Green Kit 9
    RNAspectra Green Kit 10
    RNAspectra Red Kit 1
    RNAspectra Red Kit 2
    RNAspectra Red Kit 3
    RNAspectra Red Kit 4
    RNAspectra Red Kit 5
    RNAspectra Red Kit 6
    RNAspectra Red Kit 7
    RNAspectra Red Kit 8
    RNAspectra Red Kit 9
    RNAspectra Red Kit 10

    F501
    F502
    F503
    F504
    F505
    F506
    F507
    F508
    F509
    F510
    R501
    R502
    R503
    R504
    R505
    R506
    R507
    R508
    R509
    R510

    Kit
    Kit
    Kit
    Kit
    Kit
    Kit
    Kit
    Kit
    Kit
    Kit
    Kit
    Kit
    Kit
    Kit
    Kit
    Kit
    Kit
    Kit
    Kit
    Kit

     

    PCR PRODUCT CLONING AND SEQUENCING

     

     

    1.

     PCR-TRAP Cloning System - for cloning PCR products

     

    For positive-selection cloning of PCR products through blunt-end ligation. Allows direct cloning of PCR products without purification.
    This system uses a third generation cloning vector that features positive selection for cloning PCR products. Only recombinant plasmids confer antibiotic resistance, making PCR-TRAP extremely efficient. There is no need for any post-PCR manipulation before cloning, since a significant fraction of PCR products do not contain the 3' overhanging A (Clark, 1988, Nucleic Acids Res. 16:9677) The mechanism of this unique cloning system involves the phage Lambda repressor gene, cI, which has been incorporated into the PCR-TRAP Vector. When transcribed, the gene codes for a repressor protein which binds to the Lambda right operators Or1 to Or3 of the cro gene, turning off the promoter which drives the TetR gene on the plasmid. Therefore, cloning the PCR product directly into the cI gene leads to the inactivation of the repressor gene and thus the expression of the TetR gene. So the PCR-TRAP Cloning System is indeed a cloning TRAP! request info

    Cat #

    Quantity

    P404

    Kit

     

     

    2.

     PCR-TRAP Vector Primer Kit - for cloning PCR products

     

    This kit contains some of the essentail components of the PCR-TRAP Cloning System. Other components such as T4 DNA ligase, Component cells, and Tetracycline are not provided. request info

    Cat #

    Quantity

    P405

    Kit

     

    http://www.wak-chemie.net/images/spacer.gif

    3.

     Riboprobe Primer Set - for creating Riboprobe templates

     

    For PCR amplification of cDNAs cloned into the PCR-TRAP® system for riboprobe preparation.
    This reaction creates a template for riboprobe synthesis by placing the T7 and SP6 promoter sites adjacent to the insert. request info

    Cat #

    Quantity

    P406

    Set

     

    http://www.wak-chemie.net/images/spacer.gif

    4.

     ReversePrime™ cDNA Labeling Kit

     

    For labeling cDNA probes for use in "Reverse Northern" blots.
    ReversePrime Kit provides a complete system for labeling cDNA for use in "Reverse Northern" blots to conduct differential screening of positive clones generated by differential display. This kit is ideal for either dot blot or colony hybridization screening of differential display PCR products cloned into the PCR-TRAP® Cloning System. request info

    Cat #

    Quantity

    R701

    Kit

     

     

    5.

     HotPrime™ DNA Labeling Kit

     

    For optimized labeling of differential display fragments with radioactively labeled dATP.
    The HotPrime DNA labeling kit features three unique improvements over the traditional "random priming" method which allow for radioactive labeling of DNA probes with at least 5-10X more specific radioactivity. It is designed to efficiently label cDNA probes isolated from differential display for Northern or Southern blot analysis as well as library screening. However, the HotPrime Kit can also label DNA probes for any other application to a similar high specific activity. request info

    Cat #

    Quantity

    H501

    Kit

     

     

    6.

     AidSeq™ Primer Sets - for sequencing DNA products

     

    AidSeq™ Primer Set A
    Primer pair for sequencing DNA cloned into vectors such as TA cloning vector (Invitrogen) and pGEM (Promega) that contain SP6 and T7 promoter binding sites. request info

    Cat #

    Quantity

    P201

    50 reactions

    AidSeq™ Primer Set B
    Primer pairs for Sequencing DNA cloned into M13 and pUC related vectors such as pBlueScript (Stratagene). request info

    Cat #

    Quantity

    P202

    50 reactions

    AidSeq™ Primer Set C
    Primer pairs for Sequencing DNA clined into PCR-TRAP® cloning vector. request info

    Cat #

    Quantity

    P203

    50 reactions

     

     

    AP-TAG PRODUCTS

     

     

    1.

     AP-TAG CLONING SYSTEMS

     

    For non-radioactive detection of receptor/ligand interaction.

    AP-TAG Kit A
    This is the second generation of AP-TAG technology. A secreted ligand or soluble receptor can be fused with secreted alkaline phosphatase (AP) at either its N- or C-terminus to produce an "AP-body". The resulting AP fusion protein can be expressed as a secreted protein and used directly as highly sensitive affinity agents much like an antibody. request info

     

    Cat #

    Quantity

    AP-TAG Kit A - Academic
    AP-TAG Kit A - Industry

    Q201
    Q201P

    Kit
    Kit

    AP-TAG Kit B
    This single vector system is the third generation AP-TAG technology. A secreted ligand or soluble receptor can be fused with secreted alkaline phosphatase (AP) at either its N- or C-terminus to produce an "AP-body". The resulting AP fusion protein can be expressed as a secreted protein and used directly as highly sensitive affinity agents much like an antibody. The epitope tags (6xHis and myc) allow easy purification, detection and interaction assays (IP) of the AP-fusion proteins. request info

     

    Cat #

    Quantity

    AP-TAG Kit B - Academic
    AP-TAG Kit B- Industry

    Q202
    Q202P

    Kit
    Kit

     

     

    2.

     SUPER-COMPETENT CELLS

     

    For Transformation of AP-TAG Cloning Vectors.

    GH2/P3 Supercompetent Cells
    For transformation of pAPtag-2 and pAPtag-4 vectors.
    The pAPtag-2 and pAPtag-4 AP-fusion cloning vectors contain the supF gene which confers both ampicillin and tetracycline resistance when transformed into the GH2/P3 Supercompetent Cells. pAPtag-2 and pAPtag-4 vectors will not confer antibiotic resistance in an E. coli host which does not contain the P3 episome. request info

    Cat #

    Quantity

    T601

    2 mL

    AT Antibiotics Mix
    For selection of pAPtag-2 and pAPtag-4 plasmids.
    Each tube of AT antibiotics mix is conveniently packaged to prepare ampicillin (25 mg/mL) and tetracycline (10 mg/mL) plates for 1 L of LB-agar. request info

     

    Cat #

    Quantity

    AT Antibiotics Mix (1000X)

    Q601

    1 mL

    GH Competent Cells
    For transformation of pAPtag-5 vector
    The pAPtag-5 AP fusion cloning vector contains the ampicillin resistance gene. It can be easily and efficiently transformed and propagated in GH Competent cells. request info

    Cat #

    Quantity

    L301

    6 x 0.5 mL

     

    http://www.wak-chemie.net/images/spacer.gif

    3.

     ANTIBODIES AGAINST ALKALINE PHOSPHATASE

     

    AP Western Blot Kit
    For immunoblotting of AP fusion proteins.
    This kit contains the AP Antibody (rabbit polyclonal) which is specific to human placental secreted AP as well as two controls for AP Western blots. request info

    Cat #

    Quantity

    Q310

    Kit

    AP Antibody (human placenta) - Monoclonal
    For ELISA Assay of Alkaline Phosphatase or IP (Immunoprecipitation).
    This Monoclonal AP Antibody (human placental) is purified IgG 2a with a concentration of approximay 2.3 mg/mL. It is purified by DEAE chromatography and is in 15mM potassium phosphate buffer, 150mM sodium chloride, 0.1% sodium azide, pH 7.2. request info

    Cat #

    Quantity

    Q320
    Q321

    100 µg
    1 mg

    Monoclonal AP Antibody-Sepharose Beads
    For one-step purification of AP-fusion proteins and proteins interacting with AP-fusion proteins. request info

    Cat #

    Quantity

    Q330
    Q331
    Q332

    100 µL
    500 µL
    1 mL

    Antigen Elution Solution
    For eluting AP fusion protein from the Monoclonal AP Antibody-Sepharose Beads.
    This specially formulated elution buffer can break the extremely tight interaction between the antibody-antigen, allowing up to 60% recovery of the bound antigen. request info

    Cat #

    Quantity

    Q340
    Q341

    10 mL
    50 mL

     

    http://www.wak-chemie.net/images/spacer.gif

    4.

     CELL LINES FOR AP-TAG VECTOR TRANFECTION AND AP-BODY  SECRETION

     

    293T Cells
    For transfection with pAPtag vectors.
    293T is a human embryonic kidney cell line commonly used for transfection assays. Due to the expression of the large T antigen in the cell, plasmids with SV40 origin of replication (such as pAPtag-2, pAPtag-4, and pAPtag-5) can be transiently transfected and give extremely high levels of expression of AP fusion proteins (e.g. ligand-AP fusion proteins). request info

    Cat #

    Size

    Q401

    5 x 106 Cells/Vial

    293T/pAPtag-4 Stable Cell Line
    For production of high levels of AP alone.
    The 293T/pAPtag-4 stable cell line is used to produce high levels of secreted human placental alkaline phosphatase (AP) which can be used as a negative control for a ligand-AP or soluble receptor-AP fusion protein in cell surface binding assays or cell staining. request info

    Cat #

    Size

    Q402

    5 x 106 Cells/Vial

    Co-transfection Vectors
    For use as a selectable marker for transfection of cultured mammalian cells.
    The pSV-Hygro vector confers hygromycin resistance and the pBabe-Puro vector confers puromycin resistance when co-transfected into cells. request info

     

    Selectable Marker

    Cat #

    Quantity

    pSV-Hygro co-transfection vector
    pBabe-Puro co-transfection vector

    Hygromycin
    Puromycin

    Q455
    Q456

    10 µg
    10 µg

     

     

    5.

     RECEPTOR/LIGAND EXPRESSION CLONING

     

    pMT21-Neo Mammalian Expression Cloning Vector
    For construction of expression cDNA libraries.
    This cloning vector has been used extensively to construct mammalian expression cDNA libraries. The vector contains an SV40 origin of replication and the major adeno late promoter (PMAL) in front of Neo resistance cDNA insert flanked by an EcoR I and Xho I site. Using the Stratagene cDNA Synthesis Kits generally results in cDNA ends with EcoR I and Xho I sites, which can be directionally cloned into the pMT21-NeoR vector. request info

    Cat #

    Quantity

    Q454

    20 µg

    Expression Cloning Kit
    For expression cloning of cell surface receptor/ligand using AP fusion proteins (AP-bodies).
    This kit consists of a cos-1 host cell line (commonly used for expression cloning by panning) and a positive control receptor/ligand-AP pair. request info

    Cat #

    Quantity

    Q450

    20 µg

     

     

    6.

     AP ACTIVITY ASSAY REAGENTS AND BUFFERS

     

    AP Assay Reagent A
    For AP Activity Assay.
    The AP assay reagent A is formulated specifically for measuring the enzymatic activity of the alkaline phosphatase (AP).

    Cat #

    Quantity

    Q501

    10 mL

    AP Assay Reagent S
    For Cell Staining.
    The AP Assay Reagent S is formulated specifically for cell staining or Western blotting analysis of enzymatic activity of the alkaline phosphatase (AP).

    Cat #

    Quantity

    Q502

    10 mL

    HBHA Wash Buffer
    For Receptor Binding Assay.
    HBHA wash buffer consists of Hank's balanced salt solution with 0.5 mg/mL BSA and 20 mM HEPES, pH 7.0. This buffer has been used extensively for ligand-receptor binding assays.

    Cat #

    Quantity

    Q503S
    Q503L

    100 mL
    500 mL

    Cell Lysis Buffer
    For Cell Lysis in Receptor Binding Assay .
    The cell lysis buffer is used in ligand-receptor binding assay. This buffer allows rapid lysis of the cells and removal of cell nuclei before bound AP activity is measured.

    Cat #

    Quantity

    Q504

    100 mL

     

     

    重慶市華雅干細胞技術有限公司在倉庫備有現貨,歡迎新老客戶:            

     


    產品咨詢

    留言框

    • 產品:

    • 您的單位:

    • 您的姓名:

    • 聯系電話:

    • 常用郵箱:

    • 省份:

    • 詳細地址:

    • 補充說明:

    • 驗證碼:

      請輸入計算結果(填寫阿拉伯數字),如:三加四=7
    推薦產品

    2025 版權所有 © 重慶市華雅干細胞技術有限公司  備案號:渝ICP備14000349號-4 sitemap.xml 管理登陸 技術支持:化工儀器網

    地址:重慶市江北區金渝大道153號8棟20-14 傳真:023-63419626 郵件:sales@ys-bio.com

    重慶市華雅干細胞技術有限公司主要經營干細胞研究 干細胞治療產品 生物試劑 實驗耗材 藥物研發等產品。

    關注我們

    服務熱線

    400-021-2200

    掃一掃,關注我們

    主站蜘蛛池模板: 综合精品欧美日韩国产在线| 久久精品国产一区二区电影| 99久久99久久精品国产片| 欧美日韩国产精品系列| 亚洲永久永久永久永久永久精品| 精品国产综合成人亚洲区| 欧美日韩精品一区二区三区不卡| 亚洲综合av永久无码精品一区二区 | 亚洲国产精品自产在线播放| 精品人妻中文字幕有码在线| 少妇伦子伦精品无码STYLES| 国产精品久久国产精品99盘| 精品一卡2卡三卡4卡免费视频| 免费人欧美日韩在线精品| 四虎永久在线精品免费一区二区| 国产99视频精品免视看7| 久久久久亚洲精品男人的天堂| 国产精品国产高清国产专区| 2021最新国产精品网站| 亚洲精品无码MV在线观看| 亚洲午夜精品一级在线播放放| 下载天堂国产AV成人无码精品网站| 国自产精品手机在线观看视频| 国产精品免费看久久久香蕉| 日韩精品一区二区午夜成人版| 国产在线精品福利大全| 亚洲国产精品成人久久蜜臀| 99re国产精品视频首页| 精品欧美一区二区在线看片| 国产精品日韩欧美久久综合| 四虎永久在线精品国产免费 | 久久亚洲国产成人精品无码区| 欧美日韩精品系列一区二区三区 | 久久99精品久久久久久| 欧美黑人巨大精品| 欧美日韩精品| 99re久久精品国产首页2020| 国产成人精品综合在线观看| 无码8090精品久久一区| 国产精品白丝AV网站| 精品久久国产一区二区三区香蕉|