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    Anti-FOXP2 antibody (ab16046)抗FOXP2蛋白 抗體

    簡要描述:Anti-FOXP2 antibody (ab16046)抗FOXP2蛋白抗體
    貨號:ab16046
    廠家:Abcam公司
    規(guī)格:100ug
    保存溫度:-20℃ 和 2~8℃
    貨期:現(xiàn)貨2~5天,期貨2-3周

    • 產(chǎn)品型號:ab16046
    • 廠商性質(zhì):代理商
    • 更新時間:2025-02-12
    • 訪  問  量:1393
    詳細(xì)介紹

    咨詢:  (重慶) (貴陽)

    Anti-FOXP2 antibody (ab16046)抗FOXP2蛋白抗體產(chǎn)品信息:

    • Product nameAnti-FOXP2 antibody
    • Description

      Rabbit polyclonal to FOXP2

    • Tested applicationsWB, IHC-FoFr, IHC-Fr, ICC/IF, IHC-P
    • Species reactivity

      Reacts with: Mouse, Rat, Human

    • Immunogen

      Synthetic peptide conjugated to KLH derived from within residues 700 to the C-terminus of Human FOXP2.

      Read Abcam's proprietary immunogen policy

      (Peptide available as ab16278.)

    • Positive control
      • This antibody gave a positive signal in HEK293 Whole Cell Lysate

    Properties

    • FormLiquid
    • Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
    • Storage bufferPreservative: 0.02% Sodium Azide
      Constituents: 1% BSA, PBS, pH 7.4
    • Concentration 100 µg at 1 mg/ml

    • PurityImmunogen affinity purified
    • Clonality Polyclonal
    • IsotypeIgG
    • Research Areas
      • Epigenetics and Nuclear Signaling
      • Transcription
      • Domain Families
      • Forkhead Box
      • Epigenetics and Nuclear Signaling
      • Transcription
      • Domain Families
      • Forkhead Box
      • FOXP

    Applications

    Our Abpromise guarantee covers the use of ab16046 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    ApplicationNotes
    WBWB: 1/1000. Can be blocked with Human FOXP2 peptide (ab16278). (see abreview)
    IHC-FoFrIHC-FoFr: Use at an assay dependent dilution. PubMed: 19490899
    IHC-FrIHC-Fr: Use at an assay dependent dilution.
    ICC/IFICC/IF: Use at an assay dependent dilution.
    IHC-PIHC-P: 1/50 - 1/2500. PubMed: 19136970

    Target

    • FunctionTranscriptional repressor that may play a role in the specification and differentiation of lung epithelium. May also play a role in developing neural, gastrointestinal and cardiovascular tissues. Can act with CTBP1 to synergistically repress transcription but CTPBP1 is not essential. Involved in neural mechanisms mediating the development of speech and language.
    • Tissue specificityIsoform 1 and isoform 6 are expressed in adult and fetal brain, caudate nucleus and lung.
    • Involvement in diseaseDefects in FOXP2 are the cause of speech-language disorder 1 (SPCH1) [MIM:602081]; also known as autosomal dominant speech and language disorder with orofacial dyspraxia. Affected individuals have a severe impairment in the selection and sequencing of fine orofacial movements, which are necessary for articulation. They also show deficits in several facets of language processing (such as the ability to break up words into their constituent phonemes) and grammatical skills.
      Note=A chromosomal aberration involving FOXP2 is a cause of severe speech and language impairment. Translocation t(5;7)(q22;q31.2).
    • Sequence similaritiesContains 1 C2H2-type zinc finger.
      Contains 1 fork-head DNA-binding domain.
    • Developmental stageExpressed in the brain at 15 and 22 weeks of gestation, with a pattern of strong cortical, basal ganglia, thalamic and cerebellar expression. Highly expressed in the head and tail of nucleus caudatus and putamen. Restricted expression within the globus pallidus, with high levels in the pars interna, which provides the principal source of output from the basal ganglia to the nucleus centrum medianum thalami (CM) and the major motor relay nuclei of the thalamus. In the thalamus, present in the CM and nucleus medialis dorsalis thalami. Lower levels are observed in the nuclei anterior thalami, dorsal and ventral, and the nucleus parafascicularis thalami. Expressed in the ventrobasal complex comprising the nucleus ventralis posterior lateralis/medialis. The ventral tier of the thalamus exhibits strong expression, including nuclei ventralis anterior, lateralis and posterior lateralis pars oralis. Also expressed in the nucleus subthalamicus bilaterally and in the nucleus ruber.
    • DomainThe leucine-zipper is required for dimerization and transcriptional repression.
    • Cellular localizationNucleus.
    • Information by UniProt
    • Database links

      • Entrez Gene: 93986 Human
      • Entrez Gene: 114142 Mouse
      • Entrez Gene: 500037 Rat
      • Omim: 605317 Human
      • SwissProt: O15409 Human
      • SwissProt: P58463 Mouse
      • SwissProt: P0CF24 Rat
      • Unigene: 282787 Human

      see all

    • Alternative names

      • CAG repeat protein 44 antibody
      • CAGH44 antibody
      • DKFZp686H1726 antibody

      see all

    Anti-FOXP2 antibody images

    • Immunocytochemistry/ Immunofluorescence - FOXP2 antibody (ab16046)

      Immunocytochemistry/ Immunofluorescence - FOXP2 antibody (ab16046)

      Mouse spinal cord was fixed in paraformaldehyde, blocked in 1% BSA for 30 minutes then incubated with ab16046 at 1/8000 dilution for 18 hours. This image was submitted as part of a review by Jeremy Dasen.

    • Western blot - FOXP2 antibody (ab16046)

      Western blot - FOXP2 antibody (ab16046)

      This image is courtesy of an anonymous Abreview

      See Abreview

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - FOXP2 antibody (ab16046)

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - FOXP2 antibody (ab16046)

      Image courtesy of Human Protein Atlas

      ab16046 staining FOXP2 in human testis. Paraffin embedded human testis tissue was incubated with ab16046 (1/600 dilution) for 30 mins at room temperature. Antigen retrieval was performed by heat induction in citrate buffer pH 6.

      ab16046 was tested in a tissue microarray (TMA) containing a wide range of normal and cancer tissues as well as a cell microarray consisting of a range of commonly used, well characterised human cell lines.

      Further results for this antibody can be found at www.proteinatlas.org

    • Western blot - FOXP2 antibody (ab16046)

      Western blot - FOXP2 antibody (ab16046)

      Anti-FOXP2 antibody (ab16046) at 1 µg/ml + HEK293 (Human embryonic kidney cell line) Whole Cell Lysate at 10 µg

      Secondary
      Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

      Performed under reducing conditions.

      Observed band size : 90 kDa (why is the actual band size different from the predicted?)
      Additional bands at : 56 kDa,70 kDa. We are unsure as to the identity of these extra bands.

    • Immunohistochemistry (Frozen sections) - FOXP2 antibody (ab16046)

      Immunohistochemistry (Frozen sections) - FOXP2 antibody (ab16046)This image is courtesy of an anonymous Abreview

      ab16046 staining FOXP2 in mouse brain tissue sections by IHC-Fr (Frozen sections). Tissue samples were fixed with paraformaldehyde, permeabilized by 0.4% Triton X and blocked with 10% serum for 1 hour at 22°C. The sample was incubated with primary antibody (1/8000) at 4°C for 16 hours. An Alexa Fluor®488-conjugated Goat polyclonal to mouse IgG (1/1000) was used as secondary antibody.

      See Abreview

    • Immunocytochemistry/ Immunofluorescence - FOXP2 antibody (ab16046)

      Immunocytochemistry/ Immunofluorescence - FOXP2 antibody (ab16046)

      ICC/IF image of ab16046 stained HepG2 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab16046, 1µg/ml) overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti-rabbit IgG - H&L, pre-adsorbed (ab96899) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

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