Anti-p75 NGF Receptor antibody MLR2 （FITC） （ab62122）

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Anti-p75 NGF Receptor antibody [MLR2] (FITC) (ab62122)抗神經(jīng)生長因子受體抗體產(chǎn)品信息：

• Product name：Anti-p75 NGF Receptor antibody [MLR2] (FITC)

  Mouse monoclonal [MLR2] to p75 NGF Receptor (FITC)

• ConjugationFITC. Ex: 493nm, Em: 528nm
• Conjugation notesThe antibody was conjugated to Fluorescein isomer 1 (FITC, Sigma). A minimum fluorescein: protein ratio of 3:1 is guaranteed. The conjugate was purified via gel filtration using a G25 fine grain gel in 10 mMTris/50mM NaCl solution.
• SpecificityReacts with human, mouse and rat. Other species have not yet been tested but it is expected that this antibody will be useful for the study of p75 in primates and other species.
• Tested applicationsICC/IF, Flow Cyt, ELISA
• Species reactivity

  Reacts with: Mouse, Rat, Human

• Immunogen

  Human recombinant p75

• Positive control
  • NSC34 motor neuron cell line

Properties

• FormLiquid
• Storage instructionsStore at +4°C.
• Storage bufferPreservative: None
  Constituents: PBS

• Concentration 50 µg at 1 mg/ml

• PurityProtein G purified
• Purification notesImmunoglobulin (IgG2A) was purified using Protein G column (Amersham Pharmacia), polished with Sephacryl 200HR (Amersham Pharmacia) in PBS and then lyophilized. Purity was analysed using electrophoresis, 4-12% Bis Tris Gel (Invitrogen).
• Clonality Monoclonal
• Clone numberMLR2
• IsotypeIgG2a
• Research Areas
  • Neuroscience
  • Neurology process
  • Growth and Development
  • Neurotrophins
  • Stem Cells
  • Neural Stem Cells
  • Surface Molecules
  • Stem Cells
  • Mesenchymal Stem Cells
  • Surface Molecules

Applications

Our Abpromise guarantee covers the use of ab62122 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

• Application notesIs unsuitable for ,BL or WB.

Target

• FunctionLow affinity receptor which can bind to NGF, BDNF, NT-3, and NT-4. Can mediate cell survival as well as cell death of neural cells.
• Sequence similaritiesContains 1 death domain.
  Contains 4 TNFR-Cys repeats.
• DomainDeath domain is responsible for interaction with RANBP9.
  The extracellular domain is responsible for interaction with NTRK1.
• Post-translational
  modificationsN- and O-glycosylated.
  O-linked glycans consist of Gal(1-3)GalNAc core elongated by 1 or 2 NeuNAc.
  Phosphorylated on serine residues.
• Cellular localizationMembrane.
• Information by UniProt

• Database links

  • Entrez Gene: 4804 Human
  • Entrez Gene: 18053 Mouse
  • Entrez Gene: 24596 Rat
  • Omim: 162010 Human
  • SwissProt: P08138 Human
  • SwissProt: Q9Z0W1 Mouse
  • SwissProt: P07174 Rat
  • Unigene: 415768 Human

• Alternative names

  • CD271 antibody
  • CD271 antigen antibody
  • Gp80 LNGFR antibody

Anti-p75 NGF Receptor antibody [MLR2] (FITC) images

• 

  Immunocytochemistry/ Immunofluorescence - p75 NGF Receptor antibody [MLR2] (FITC) (ab62122)

  Ab62122 (1-2 ug/ml) intracellular staining of p75NTR in NSC34 motor neuron cell line by live confocal imaging.

• 

  Flow Cytometry - p75 NGF Receptor antibody [MLR2] (FITC) (ab62122)

  Overlay histogram showing PC12 cells stained with ab62122 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab62122, 1/10 dilution) for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 2µg/1x10⁶ cells) with secondary antibody DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Acquisition of >5,000 events was performed. This antibody gave a positive signal in PC12 cells fixed with methanol (5 min) used under the same conditions.

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