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    Anti-Cyclin T1 antibody 抗細胞周期蛋白T1抗體

    簡要描述:Anti-Cyclin T1 antibody 抗細胞周期蛋白T1抗體
    貨號:ab27963
    廠家:Abcam公司
    規格:200ul
    保存溫度:-20℃ 和 2~8℃
    貨期:現貨2~5天,期貨2-3周

    • 產品型號:ab27963
    • 廠商性質:代理商
    • 更新時間:2025-02-12
    • 訪  問  量:1292
    詳細介紹

    咨詢:  (重慶) (貴陽)

    Anti-Cyclin T1 antibody 抗細胞周期蛋白T1抗體產品信息:

    • Product nameAnti-Cyclin T1 antibody
    • Description

      Sheep polyclonal to Cyclin T1

    • Tested applicationsWB, IP, EMSA
    • Species reactivityReacts with: Mouse, Horse, Human, Monkey

      Does not react with

      Fruit fly (Drosophila melanogaster)
    • Immunogen

      Recombinant fragment, corresponding to C terminal amino acids 291-726 of Human Cyclin T1

    • General notes

       

      ab27963 will also recognise truncated forms of cyclin T1 which are frequently produced by proteolysis during sample preparation.

    Properties

    • FormLiquid
    • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
    • PurityWhole antiserum
    • Primary antibody notes ab27963 will also recognise truncated forms of cyclin T1 which are frequently produced by proteolysis during sample preparation.
    • Clonality Polyclonal
    • IsotypeIgG
    • Application notesEMSA: Use at an assay dependent dilution PMID 17576689.
      IP: Use at an assay dependent dilution.
      WB: 1/1000. Detects a band of approximay 87 kDa (predicted molecular weight: 81 kDa).

      Not yet tested in other applications.
      Optimal dilutions/concentrations should be determined by the end user.
    • Target

    • FunctionRegulatory subunit of the cyclin-dependent kinase pair (CDK9/cyclin-T1) complex, also called positive transcription elongation factor B (P-TEFb), which is proposed to facilitate the transition from abortive to productive elongation by phosphorylating the CTD (carboxy-terminal domain) of the large subunit of RNA polymerase II (RNA Pol II). In case of HIV or SIV infections, binds to the transactivation domain of the viral nuclear transcriptional activator, Tat, thereby increasing Tat's affinity for the transactivating response RNA element (TAR RNA). Serves as an essential cofactor for Tat, by promoting RNA Pol II activation, allowing transcription of viral genes.
    • Tissue specificityUbiquitously expressed.
    • Sequence similaritiesBelongs to the cyclin family. Cyclin C subfamily.
    • Cellular localizationNucleus.

      Anti-Cyclin T1 antibody images

      Western blot - Cyclin T1 antibody (ab27963)

      Western blot - Cyclin T1 antibody (ab27963)

      All lanes : Anti-Cyclin T1 antibody (ab27963) at 1/1000 dilution
      Lane 1 : HeLa whole cell lysate
      Lane 2 : HeLa cell nuclear lysate
      Lane 3 : monkey Vero cell lysate
      Lane 4 : mouse kidney cell lysate
      Lane 5 : Drosophila S2 cell lysate
      Lane 6 : horse dermal cell lysate
      Secondary
      donkey anti-sheep peroxidase conjugate at 1/20000 dilution
      Predicted band size : 81 kDa
      Observed band size : 87 kDa

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